A nested PCR assay
targeting the small-subunit ribosomal RNA has been developed.1
Preliminary results indicate that the Cyclospora-specific PCR primers cross react
with some Eimeria species and the sensitivity of the assay may be low (62%).2
Agarose gel (2%) analysis of a PCR diagnostic
test for detection of Cyclospora DNA. PCR was performed using nested primers
CYCF1E/CYCR2B (first round) and CYCF3E/CYCR4B (second round).1,2
- Lane S: Molecular base
pair standard (100-bp ladder). Black arrows show the size of standard bands.
- Lane 1:
Cyclospora positive fecal specimen. The red arrow shows the diagnostic band for Cyclospora
cayetanensis (size: 308 bp).
- Relman DA, Schmidt TM, Gajadhar
A, Sogin M, Cross J, Yoder K, et al. Molecular phylogenetic analysis of Cyclospora,
the human intestinal pathogen, suggests that it is closely related to Eimeria
species. J Infect Dis 1996;173:440-45.
- Pieniazek NJ, Slemenda SB, da
Silva AJ, Alfano EM, Arrowood MJ. PCR confirmation of infection with Cyclospora
cayetanensis. Emerg Infect Dis 1996;2:357-9.