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[Plasmodium falciparum] [Plasmodium knowlesi] [Plasmodium malariae] [Plasmodium ovale] [Plasmodium vivax]

Molecular Diagnosis

A

A: Agarose gel (2%) analysis of a PCR diagnostic test for species-specific detection of Plasmodium DNA.  PCR was performed using nested primers of Snounou et al.¹

• Lane S: Molecular base pair standard (50-bp ladder).  Black arrows show the size of standard bands.
• Lane 1: The red arrow shows the diagnostic band for P. vivax (size: 120 bp)
• Lane 2: The red arrow shows the diagnostic band for P. malariae (size: 144 bp)
• Lane 3: The red arrow shows the diagnostic band for P. falciparum (size: 205 bp)
• Lane 4: The red arrow shows the diagnostic band for P. ovale (size: 800 bp)

Reference:

Snounou G, Viriyakosol S, Zhu XP, et al. High sensitivity detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 1993;61:315-320.