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Cyclosporiasis
[Cyclospora cayetanensis]

Cyclospora cayetanensis oocysts are spherical, 7.5-10 µm in diameter and unsporulated when passed in feces.  Sporulation in the environment is temperature-dependent and may take one to several weeks for an infective oocyst to contain two sporocysts, each containing two sporozoites.  Oocysts of C. cayetanensis will autofluoresce under ultraviolet (UV) microscopy.

Cyclospora oocysts, safranin

Cyclospora oocysts, safranin

A

B

A, B:  Oocysts of C. cayetanensis stained with safranin (SAF).

Cyclospora oocysts, safranin

Cyclospora oocysts, safranin

C

D

C, D:  Oocysts of C. cayetanensis stained with safranin (SAF).

Cyclospora oocysts, safranin

Cryptosporidium and Cyclospora, safranin

E

F

E:  A pair of oocysts of C. cayetanensis stained with safranin (SAF).
F: 
Oocyst of C. cayetanensis (yellow arrow) along with an oocyst of Cryptosporidium parvum (red arrow), stained with safranin (SAF).  Cryptosporidium spp. also stain with the safranin and modified acid-fast stains.

 

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