Image Library [Last Modified: ]
Amebiasis
[Entamoeba histolytica]

Mature Entamoeba histolytica/Entamoeba dispar cysts have 4 nuclei that characteristically have centrally located karyosomes and fine, uniformly distributed peripheral chromatin.  Cysts usually measure 12 to 15 µm.

E. histolytica immature cyst stained with trichrome E. histolytica/E. dispar immature cyst stained with trichrome
A B

A: Immature cyst of E. histolytica.  The specimen was preserved in poly-vinyl alcohol (PVA) and stained with trichrome.  PCR was performed on this specimen to differentiate between E. histolytica and E. dispar.
B: Immature cyst of E. histolytica/E. dispar stained with trichrome.  Image taken at 1000× oil magnification and contributed by the Kansas Department of Health and Environment.

E. histolytica/E. dispar immature cyst stained with trichrome E. histolytica/E. dispar immature cyst stained with trichrome
C D

C, D: Immature cysts of E. histolytica/E. dispar stained with trichrome.  The cysts have large vacuoles and the chromatin around the nucleus is clumpy.  The cysts range in size from 10.3 to 15.7 µm.  Images taken at 1000× oil magnification and contributed by the Kansas Department of Health and Environment.

 
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