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[Entamoeba histolytica]

Mature Entamoeba histolytica/Entamoeba dispar cysts have 4 nuclei that characteristically have centrally-located karyosomes and fine, uniformly distributed peripheral chromatin.  Cysts usually measure 12 to 15 µm.

A	B

A: Cyst of E. histolytica/E. dispar stained with trichrome.  Note the chromatoid body with blunt ends (red arrow).
B: Cyst of E. histolytica/E. dispar stained with trichrome.

C	D

C, D: Cysts of E. histolytica/E. dispar stained with trichrome.  Two to three nuclei are visible in the focal plane (black arrows), and the cysts contain chromatoid bodies with typically blunted ends (red arrows).  The chromatoid body in C is particularly well demonstrated.