Diagnostic Findings [Last Modified: ]
Free-living amebic infections
[Acanthamoeba spp.] [Balamuthia mandrillaris] [Naegleria fowleri] [Sappinia spp.]
Causal Agent Life Cycle Geographic Distribution Clinical Features Laboratory Diagnosis Treatment

Molecular diagnosis

Real-Time PCR

A real-time PCR was developed at CDC for identification of Acanthamoeba spp., Naegleria fowleri, and Balamuthia mandrillaris in clinical samples.1  This assay uses distinct primers and TaqMan probes for the simultaneous identification of these three parasites.

Click here to learn more about TaqMan real-time PCR.

References:

  1. Qvarnstrom Y, Visvesvara GS, Sriram R, da Silva AJ. Multiplex real-time PCR assay for simultaneous detection of Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri. J Clin Microbiol 2006;44(10):3589-3595.

 

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